Fig. 2.

Protection from metastasis in Ackr2^−/− mice is associated with increased numbers of monocytes and neutrophils in blood and lungs. a Absolute number of circulating inflammatory monocytes (CD45⁺/CD11b⁺/Ly6C^hi) and b neutrophils (CD45⁺/CD11b⁺/Ly6G⁺) in NeuT/Ackr2^+/+ (white squares), and NeuT/Ackr2^−/− (black squares), Balb/c WT (white triangles), and Ackr2^−/− (black triangles) mice (n = 9 for NeuT/Ackr2^+/+ and 7 for NeuT/Ackr2^−/−, 3 for both WT and Ackr2^−/− at 10 and 15 weeks; 6 for NeuT/Ackr2^+/+ and 5 for NeuT/Ackr2^−/−, 5 for both WT and Ackr2^−/− at 25 weeks). c Absolute number of neutrophils, inflammatory monocytes, alveolar (CD11b^low/F4/80⁺/Ly6C^int/CD11c⁺/Ly6G⁻) and interstitial macrophages (CD11b⁺/F4/80^int/Ly6C⁻/CD11c⁻/Ly6G⁻) in the lungs of NeuT/Ackr2^+/+ (white columns) and NeuT/Ackr2^−/− (black columns) mice at 15 weeks of age (n = 12 for NeuT/Ackr2^+/+ and 6 for NeuT/Ackr2^−/− mice). d Representative immunohistochemical images of Ly6G staining in NeuT/Ackr2^+/+ and NeuT/Ackr2^−/− lungs at 25 weeks of age. Magnification: ×20. Scale bar: 100 μm. e Quantification of Ly6G immunohistochemical images as number of DAB-positive cells on field of view (n = 9 for NeuT/Ackr2^+/+ and 8 for NeuT/Ackr2^−/− mice, respectively). f Absolute number of neutrophils and inflammatory monocytes in the BM of WT (white columns) and Ackr2^−/− (black columns) mice on day 14 after orthotopic injection of 4T1 cells (n = 4 for both WT and Ackr2^−/− mice). Data are represented as mean (SD). p value was generated using the unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001