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. 2018 Feb 15;5:4. doi: 10.1186/s40580-018-0136-3

Fig. 7.

Fig. 7

SPMNP based Subcellular fractionation: a FluorLipid-SPMNP incubated with HeLa cells for pulse 15 min and chase 240 min; b FluorDMSA-SPMNP incubated with HeLa cells for pulse 15 min and chase 240 min; c Western blot analysis for EEA1—early endosomes and Na+K+ATPase—plasma membrane marker for NH2-Lipid-SPMNP incubated with HeLa cells for pulse 15 min and chase 0, 15 and 30 min; d Western blot analysis for early endosomes and plasma membrane marker for DMSA-SPMNP incubated with HeLa cells for pulse 15 min and chase 240 min. Red Label represents the enrichment of EEA1 which is a marker for early endosomes