Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 14;8:3048. doi: 10.1038/s41598-018-21322-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

PGRMC1 knockdown drives differentiation of hPSCs into multi-lineage cells. (a) Flow cytometric analysis of early and late apoptotic cells with annexin V and PI. Control or PGRMC1 knockdown H9 hPSCs were stained with PI and annexin V-FITC. Shown is statistical analysis of single annexin V-positive (AV+) and both annexin V- and PI-positive cells (AV+PI+). Each bar represents the mean value of three independent experiments ± SD (n = 4; ns, not significant). (b) The expression levels of caspase-3, active caspase-3, p62 and LC3B in control and PGRMC1 knockdown hPSCs. β-actin was used as internal protein control and loading control. Full-length blots are presented in Supplementary Figure 8. Images are representative of at least three independent experiments. (c) Real-time PCR analysis of mRNA levels of early differentiation genes in EB of hPSCs. EBs were cultured for 7 and 10 days. The graph represents the mean values of two independent determinations ± SD. (d) Real-time PCR analysis of mRNA levels of early differentiation genes in control or PGRMC1 knockdown hPSCs. The graph represents the mean values of seven independent determinations ±SD (n = 7; *p < 0.05; **p < 0.01; ***p < 0.005). (e) Real-time PCR analysis of mRNA levels of trophectoderm genes in control or PGRMC1 knockdown hPSCs. The graph represents the mean values of 5 independent determinations ± SD (n = 5; ***p < 0.005; ns, not significant).