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. 2018 Feb 14;8:3004. doi: 10.1038/s41598-018-21399-6

Figure 2.

Figure 2

Patients with SH1 show microglial activation in molecular layer but not in white matter. Patients with more severe liver disease show microglial activation in both layers. Immunohistochemistry was performed using an antibody against Iba-1. Representative images of white matter (A) and molecular layer (C) are shown for patients with different grades of liver disease. Images showing both the molecular layer (ML, in red) and white matter (WM, in blue) at stages SH1 (E) and SH2 (F) of steatohepatitis are also shown. The perimeter of microglial cells was quantified in the molecular layer (B) and white matter (D). One-way ANOVA with Bonferroni post-hoc test was performed to compare all groups. Values are the mean ± SEM of 4–9 individuals per group. Values significantly different from controls are indicated by asterisks and from SH1 patients by a. *p < 0.05; ***p < 0.005; ap < 0.05; aap < 0.001; aaap < 0.005. bar in blue = 50 μm; bar in red = 100 μm.