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. 2018 Feb 14;8:3003. doi: 10.1038/s41598-018-21376-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Operational steps of the novel kit for rapid nucleic acid test in field settings. 1. Use cotton swabs for sampling. 2. Heat the tube for DNA denaturation. 3. Squeeze out two droplets. 4. Amplify the DNA at a constant temperature. 5. Observe the results.