Table 1.

Comparison of qPCR results of extracted nucleic acids from Mycobacterium tuberculosis.

Sample	Sample treatment method	1(Ctvalue)	2(Ctvalue)	3(Ct value)	4(Ct value)
Pure bacterial liquid inoculum	Takarakit	23.76	27.36	30.62	34.45
		24.14	27.54	30.89	33.57
	Multi-functional sample treatment tube	24.97	28.57	31.2	33.84
		25.04	28.67	31.18	34.45
Simulated sputum sample	Qiagen kit	25.33	27.7	30.69	N/A
		25.21	27.14	30.17	N/A
	Multi-functional	25.09	27.33	30.7	N/A
	sample treatment tube	24.84	27.24	31.46	N/A

Note: 1, 2, 3, and 4 denote the count of different colonies of M. tuberculosis. 1: 10⁷ CFU/ml; 2: 10⁶ CFU/ml; 3: 10⁵ CFU/ml, 4: 10⁴ CFU/ml. Each sample had a parallel control.