Figure 1.

Xyloglucan (XG) secretion from plants as determined by enzyme‐linked immunosorbent assay (ELISA) with XG MAb LM25 (commercial XG equivalents). (a) Survey of XG release from a range of angiosperms and bryophytes. Most angiosperms were grown in a hydroponic system (H) for 14 d and FW and XG in hydroponate were assessed. Arabidopsis and bryophytes were grown in shaking liquid culture (LC) for 28 d and FWs and XG in media were assessed. In the case of Marchantia polymorpha and Lunularia cruciata, gemmae were grown on solid media (agar, water) for 28 d and the gemmae were removed and agar extracted for ELISA assessments. Results are presented as XG (g g⁻¹ FW). n = 3; error bars, + SD. (b) Wheat seedlings grown hydroponically (eight seedlings, aerated 9 l medium) and growth medium samples taken at indicated time points for ELISA assessment. M. polymorpha gemmae were placed individually on agar solid media and at time points gemmae were removed, and agar plugs were taken and extracted with 1 ml water for ELISA assessments. Results are presented as XG released per plant at shown time points. Error bars, + SD.