Table 1.

Screening of SS-variants.

Enzyme	Mutated residues	λ	R2	p-value
WT	—	0.00151	0.953
SS01*	S10, H216	0.00162	0.965	0.0407
SS02*	A29, L179	0.00201	0.996	0.0013
SS03	S67, P121	0.00151	0.995	0.8234
SS04*	A74, I399	0.00242	0.997	0.0004
SS05	Y95, A168	0.00187	0.993	0.8326
SS06*	G100, P260	0.00202	0.960	0.0028
SS07*	H106, G150	0.00115	0.997	0.0022
SS08*	K438, E441	0.00138	0.984	0.0187
SS09*	F148, W151	0.00181	0.972	0.0056
SS10*	V202, L263	0.00063	0.426	0.0021
SS11*	K233, P404	0.00134	0.924	0.0202
SS12*	K234, A407	0.00183	0.999	0.0027
SS13*	Q247, S337	0.00173	0.990	0.0070
SS14*	M248, V253	0.00202	0.939	0.0036
SS15*	C290, S304	0.00221	0.926	0.0026
SS16*	G432, Q435	0.00166	0.888	0.0475
SS17*	D295, K303	0.00118	0.993	0.0027
SS18*	R298, Q301	0.00187	0.999	0.0022
SS19*	L324, A332	0.00170	0.997	0.0080

As a result of the comparison of the λ value for the 19 SS-variants with λ_WT, four mutants (SS03, SS07, SS08, SS17) were selected for experimental production and characterization. Statistically significant differences between the λ value of SS-variant and λ of WT are marked with (*).