Table 2.

Comparison of the apparent steady state parameters for wild type Amadoriase I to the mutated enzymes using fructosyl-lysine as substrate.

Enzyme	Km [mM]	kcat [s−1]	kcat/Km [s−1 mM−1]
WT	0.51 ± 0.19	21.55 ± 3.08	41.68 ± 16.48
SS03	0.34 ± 0.13	21.90 ± 2.73	64.17 ± 25.5
SS07	0.99 ± 0.22	11.97 ± 1.69	12.02 ± 2.86
SS17	0.68 ± 0.18	22.91 ± 2.12	33.34 ± 10.16
SSDM	0.97 ± 0.22	14.50 ± 1.67	14.85 ± 3.85

Experiments were performed at 25 °C, as described in the material and methods section.