Fig. 3.

ChinZIKV elicits ZIKV-specific humoral and cellular immune responses in non-human primates. a, b Monkeys were immunized s.c. with a single dose of ChinZIKV (n = 5) or PBS as a control (n = 3). ZIKV-specific IgG and neutralizing antibody titers were determined by ELISA and PRNT₅₀, respectively. Dotted lines indicate the limits of detection. c PBMCs from immunized monkeys were collected at the indicated times. The production of IFN-γ by PMBCs stimulated with the ZIKV E protein was measured by ELISPOT assay and expressed as spot-forming units (SFU) per 10⁶ PBMCs. Experiments were performed in duplicate (error bars represent SD). Significance was calculated using a one-way ANOVA with multiple comparison tests (*, P-value < 0.05; **, P-value < 0.01). d Heat map of the cytokine profile in sera from the monkeys inoculated with ChinZIKV or ZIKV at day 7 post inoculation. Each sample was analyzed using a Monkey Cytokine Magnetic 29-Plex Panel kit. Each cytokine level is summarized as the log₁₀ of the ratio relative to baseline (Day 0 post infection)