Table 2.
Summary of sequence alignments for LegHb and the 17 host proteins identified in AllergenOnline searches.
| Query protein | AOL results | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Full FASTA | 80 mer | 8 mer | ||||||||
| No. of matches | Matched allergend) | Number of hits ID > 35% | Best IDe) | Matched allergen | Number of hits | |||||
| Number a) | Protein name (GI#) [Accession] | Number of AA b) | E score | IDc) >50% | ||||||
| <10 | <1 | |||||||||
| n/a | LegHb (126241) [P02236.2] | 145 | 20+ | 12 | 0 | n/ae) | 0 | n/a | n/a | 0 |
| 1 | Alpha aminoadipate reductase (238030060) [CAY67983.1] | 1400 | 4 | 0 | 0 | n/a | 0 | n/a | n/a | 0 |
| 2a | Cobalamin‐independent methionine synthase (238030843) [CAY68766.1] | 768 | 12 | 1 | 0 | Sal k 3 | 630 | 77.5% | Sal k 3 | 21 |
| 2b | Aconitase (254564667) [XP_002489444.1] | 780 | 14 | 2 | 0 | n/a | 0 | n/a | n/a | 0 |
| 3 | Transketolase (238030057) [CAY67980.1] | 679 | 20+ | 0 | 0 | n/a | 0 | n/a | n/a | 0 |
| 4 | Glycerol kinase (238034027) [CAY72049.1] | 621 | 20+ | 0 | 0 | n/a | 0 | n/a | n/a | 0 |
| 5a | Catalase A (254569930) [XP_002492075.1] | 510 | 20+ | 4 | 0 | Pen c 30 | 214 | 60% | n/a | 0 |
| 5b | G6PD (238031000) [CAY68923.1] | 504 | 6 | 1 | 0 | Bla g 3 | 10 | 37% | n/a | 0 |
| 5c | Hypothetical protein PAS (238031215) [CAY69138.1] | 525 | 20+ | 8 | 2 (Cla h 10, Alt a 10) | Cla h 10 | 442 | 72.5% | Cla h 10 | 41 |
| Alt a 10 | 442 | 72.5% | Alt a 10 | 27 | ||||||
| Lep d 13 | 1 | 35.4% | ||||||||
| 6 | Mitochondrial ALDH (238033249) [CAY71271.1] | 501 | 20+ | 8 | 2 (Cla h 10, Alt a 10) | Cla h 10 | 422 | 76.2% | Cla h 10 | 41 |
| Alt a 10 | 422 | 76.2% | Alt a 10 | 25 | ||||||
| 7a | Delta‐aminolevulinate dehydratase (238033645) [CAY71667.1] | 341 | 9 | 0 | 0 | n/a | 0 | n/a | n/a | 0 |
| 7b | Mitochondrial alcohol dehydrogenase isozyme III (238031179) [CAY69102.1] | 350 | 12 | 1 | 1 (Cand a 1) | Cand a 1 | 271 | 85% | Cand a 1 | 49 |
| 7c | Malate dehydrogenase (238034064) [CAY72086.1] | 342 | 18 | 4 | 1 (Mala f 4) | Mala f 4 | 216 | 70% | Yeast Mala f 4 | 25 |
| Pis s 2 | 7 | 36.2% | ||||||||
| 7d | Putative protein, unknown function (238033788) [CAY71810.1] | 328 | 10 | 1 | 0 | n/a | 0 | n/a | n/a | 0 |
| 8 | Triose phosphate isomerase (238032989) [CAY71012.1] | 248 | 10 | 5 | 4 (Tri a 31, two isoforms of Der f 25, Cra c 8) | Tri a 31 | 169 | 62.5% | Der f 25 | 17 |
| Der f 25.0101 (isoform) | 169 | 60.0% | Der f 25 | 17 | ||||||
| Der f 25.0201 (isoform) | 169 | 60.0% | Cra c 8 | 11 | ||||||
| Cra c 8 | 169 | 57.5% | Tri a 31 | 17 | ||||||
| 9a | Hypothetical protein (cyclophilin) PP7435 (328350030) [CCA36430.1] | 161 | 17 | 7 | 7 (PPIasef) from fungus: Asp f 27, Der f 29, Mala s 6, Cat r 1, PPIase from birch: Bet v 7, from carrot Daucus carota, and from Fungus: Asp f 11) | Mala s 6 | 82 | 87.5% | Asp f 27 | 23 |
| Asp f 27 | 82 | 85% | Der f 29 | 23 | ||||||
| Cat r 1 | 82 | 81.3% | Bet v 7 | 11 | ||||||
| Der f 29 | 82 | 80% | Cat r 1 | 19 | ||||||
| Asp f 11 | 82 | 80% | (Unassigned by IUIS) PPIase from Dauces carota | 10 | ||||||
| Bet v 7 | 82 | 80% | Mala s 6 | 31 | ||||||
| (Unassigned by IUIS) PPIase from Dauces carota | 82 | 78% | Asp f 11 | 17 | ||||||
| 9b | Cytosolic superoxide dismutase (238034030) [CAY72052.1] | 154 | 20+ | 23 | 23 (isoforms of Ole e 5) | Ole e 5 23 isoforms | 75 | 60 to 55% | n/a | 0 |
| 10 | Mitochondria ATPase inhibitor (238029769) [CAY67692.1] | 84 | 17 | 4 | 0 | n/a | 0 | n/a | n/a | 0 |
a) Protein band numbers correspond to the numbers labeled on the stained gel in Figure 1;
b) AAs, amino acids;
c) ID, identity (%);
d) Allergen name in IUIS allergen list unless denoted as unassigned by IUIS;
e) n/a, not available or no answer.
f) PPIase, peptidylprolyl isomerase.