Figure 1. ATG16L1 is recruited to endolysosomal membranes during non‐canonical autophagy.

• A
  Confocal images of control and starved HCT116 cells expressing GFP‐LC3 and stained for ATG16L1. Arrows indicate autophagosome puncta double positive for LC3 and ATG16L1. Scale bar: 10 μm.
• B–D
  Confocal images of ATG16L1 and GFP‐LC3 on (B) zymosan‐containing phagosomes in J774A.1 cells (arrows indicate phagosomes), scale bar: 5 μm; (C) monensin‐treated entotic corpse vacuoles in MCF10A cells (asterisk indicate entotic corpse, arrows indicate entotic vacuoles), scale bar: 10 μm; and (D) latex bead‐containing phagosomes in monensin‐treated HCT116 cells (asterisk indicate bead‐containing phagosomes, arrows indicate phagosome membranes), scale bar: 5 μm.
• E, F
  Confocal images of GFP‐LC3 in (E) starved cells or (F) entotic corpse vacuoles in monensin‐treated MCF10A cells ± wortmannin pretreatment. Scale bars: 10 μm.
• G
  Western blotting of LC3 in control, starved or monensin‐treated HEK293 cells ± wortmannin.
• H
  Quantification of LC3‐II/LC3‐I ratios from (G).
• I, J
  Confocal images of WIPI2b staining and GFP‐LC3 in (I) starved HCT116 cells. Arrows indicated double‐positive autophagosome structures, and (J) entotic corpse vacuoles in monensin‐treated MCF10A cells. Scale bars: 10 μm.

Data information: In (H), data are presented as mean + SEM from three separate experiments. *P < 0.04 (Student's t‐test).