Figure EV1. The WD40 CTD of ATG16L1 is not required for canonical autophagy.

1. Confocal images of GFP‐LC3‐expressing HCT116 ATG16L1 ^−/− complemented with full‐length (FL), ΔFBD or ΔWD ATG16L1 under fed or starvation conditions. Scale bar: 10 μm.
2. Quantification of GFP‐LC3 puncta in ATG16L1‐complemented HCT116 cells under fed or starvation conditions.

Data information: In (B), data are presented as mean ± SEM from three independent experiments. ***P < 0.0004, **P < 0.002 (Student's t‐test).