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. 2018 Jan 9;37(4):e97840. doi: 10.15252/embj.201797840

Figure EV2. Monensin‐induced LC3 lipidation to entotic corpse vacuole requires the WD40 CTD of ATG16L1.

Figure EV2

  1. Quantification of GFP‐LC3 recruitment to LAMP1‐positive entotic corpse‐containing vacuoles in MCF10A ATG16L1 −/− cells complemented full‐length (FL), ΔFBD or ΔWD ATG16L1 ± monensin (100 μM, 1 h).
  2. Representative confocal images of entotic corpse‐containing vacuoles in GFP‐expressing MCF10A cells treated with 100 μM monensin for 1 h and stained for LAMP1 (red) and DNA (blue). Scale bar: 10 μm.
  3. Representative sequence images from FRAP analysis of GFP‐LC3 on entotic corpse‐containing vacuoles treated with monensin (100 μM, 1 h). The region marked by a broken‐line circle was photobleached, and the recovery of fluorescence at line 1 and 2 was monitored. Scale bar: 10 μm.
  4. Quantification of GFP fluorescence at line 1 and 2 from (C).
Data information: In (A), data are presented as mean ± SEM from three independent experiments.