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. 2018 Jan 9;37(4):e97840. doi: 10.15252/embj.201797840

Figure EV5. ATG16L1 T300A mutation has no effect on non‐canonical autophagy‐associated LC3 lipidation.

Figure EV5

  1. Wide‐field time‐lapse images showing the recruitment of GFP‐LC3 to entotic vacuoles in wild‐type HCT116 cell‐in‐cell structures. Arrow indicates GFP‐LC3 recruitment to entotic vacuole. Asterisks indicate the host and internalised cells.
  2. Quantification of GFP‐LC3 recruitment to entotic vacuoles over 20 h in wild‐type and ATG16L1 T300A‐expressing HCT116 cells. Data represent analysis of > 15 structures.
  3. Confocal images of latex bead‐containing phagosomes in monensin‐treated (100 μM, 1 h) GFP‐LC3‐expressing HCT116 cells expressing wild‐type or T300A ATG16L1. Asterisks indicate latex bead‐containing phagosomes, and arrows indicate GFP‐LC3‐positive phagosomes.
  4. Quantification of GFP‐LC3 recruitment to LAMP‐1‐positive phagosomes in wild‐type and T300A HCT116 cells. 100 phagosomes were counted per experiment.
Data information: In (D), data are presented as mean ± SEM from three independent experiments.