Table 1.

Primers for site‐directed mutagenesis of human ATG16L1 (623 amino acid isoform)

Mutant	Forward
E324A For	CGCATGACGGAGCGGTCAACGCAGTG
E324A Rev	CACTGCGTTGACCGCTCCGTCATGCG
N326A For	CATGACGGAGAGGTCGCCGCAGTGCAGTTCAG
N326A Rev	CTGAACTGCACTGCGGCGACCTCTCCGTCATG
M342A For	GCCACTGGAGGCGCGGACCGCAGGGTG
M342A Rev	CACCCTGCGGTCCGCGCCTCCAGTGGC
N386A For	CTTACCTATTAGCAGCTTCAGCTGATTTTGCAAGCCGAATC
N386A Rev	GATTCGGCTTGCAAAATCAGCTGAAGCTGCTAATAGGTAAG
K410A For	GGCCACAGCGGGGCAGTCCTCTCTGCC
K410A Rev	GGCAGAGAGGACTGCCCCGCTGTGGCC
L412A For	CACAGCGGGAAAGTCGCCTCTGCCAAGTTCC
L412A Rev	GGAACTTGGCAGAGGCGACTTTCCCGCTGTG
H428A For	GATTGTCTCAGGAAGTGCCGACCGGACCCTCAAAC
H428A Rev	GTTTGAGGGTCCGGTCGGCACTTCCTGAGACAATC
N453A For	GCAGGATCCAGCTGCGCTGACATTGTTTGCAC
N453A Rev	GTGCAAACAATGTCAGCGCAGCTGGATCCTGC
F467A For	GTGTAATGAGTGGACATGCTGACAAGAAAATTCGTTTCTG
F467A Rev	CAGAAACGAATTTTCTTGTCAGCATGTCCACTCATTACAC
K490A For	GATGAACTGTTAGGGGCGATCACTGCTCTGGAC
K490A Rev	GTCCAGAGCAGTGATCGCCCCTAACAGTTCATC
D536A For	CAAATGCGGCTCTGCCTGGACCCGGGTTG
D536A Rev	CAACCCGGGTCCAGGCAGAGCCGCATTTG
N581A For	CAGCTCTTCTATCGCTGCGGTGGCGTGGG
N581A Rev	CCCACGCCACCGCAGCGATAGAAGAGCTG