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. 2018 Jan 29;37(4):e97311. doi: 10.15252/embj.201797311

Figure EV2. Porcn inhibition does not affect Evi transcription.

Figure EV2

  • A
    Wild‐type or stable Wnt3‐ and Wnt5B‐expressing HEK293T cells were treated with 5 μM LGK974 for 48 h, and AXIN2 and Evi mRNA levels were quantified by qRT–PCR. Results are shown as mean ± s.d. from three independent experiments.
  • B
    Wild‐type (wt), PorcnKO1.2, and PorcnKO1.4 HEK293T cells were transfected with the indicated overexpression constructs and analyzed in Wnt Luciferase activity assays. Results are representative of three independent experiments and shown as mean of seven technical replicates ± s.d.
  • C, D
    HCT116 (C) or A375 cells (D) were treated with 5 μM LGK974 for the indicated hours (h). Evi mRNA was quantified by qRT–PCR and normalized to GAPDH. Results are shown as mean ± s.d. from three independent experiments.

Source data are available online for this figure.