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. 2017 Nov 9;115(3):586–596. doi: 10.1002/bit.26470

Figure 5.

Figure 5

Effect of transketolase cofactors, thiamine pyrophosphate (ThDP) and MgCl2, on the transaminase activity. Activity was determined through photometric detection of acetophenone formation at 280 nm and given in mMAP min−1. The reactions were performed at 20°C with initial concentrations of 10 mM sodium pyruvate and 10 mM (S)‐α‐methylbenzylamine (MBA). Experiments were performed in quadruplicates (n = 4) and error bars represented ± 1 standard deviation about the mean