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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: Acta Biomater. 2017 Jun 10;58:302–311. doi: 10.1016/j.actbio.2017.06.016

Figure 5. Effect of material concentration and stiffness on ECM deposition.

Figure 5

BMSCs were seeded at 20 × 106 cells/mL in scaffolds of different polymer concentrations and material properties. (A) Mechanical strength post fabrication and after 4 weeks of culture. Mechanical properties of PDLLA and PLLA-PEG 1000 were significantly higher at all polymer concentrations and timepoints than corresponding PDLLA-PEG 4000 (p<0.001). (B) Cell number measured on the basis of DNA content in constructs. (C,E) Total ECM deposition measured by (C) GAG and (E) Hydroxyproline production per construct. (D, F) ECM deposition normalized to DNA content. *, p<0.001, as compared to other materials at same concentration. **, p<0.001, for main effect of material as compared to others. #, p<0.05, for main effect of material concentration between concentrations and p<0.005 for main effect of PDLLA-PEG 4000 versus other groups. †, p<0.005, for main effect of PDLLA-PEG 4000 versus other groups. All effects were determined by Tukey’s HSD post-hoc testing following two-way independent ANOVA analysis. n = 6 replicates per group.