Interaction analysis of OsMADS5 and OsMADS34 with other floral homeotic MADS-box proteins. Yeast two-hybrid assay shows the interaction patterns of rice LOFSEP members OsMADS5 and OsMADS34 with class A (OsMADS14 and OsMADS15), class B (OsMADS2, OsMADS4 and OsMADS16), class C (OsMADS3 and OsMADS58), class D (OsMADS13), AGL6-like (OsMADS6), SEP3-like (OsMADS7 and OsMADS8), and LOFSEP-like (OsMADS1, OsMADS5, and OsMADS34) proteins, respectively. The transformants were grown on selective Minimal Synthetic Dropout (sd) media at high stringency (SD/-Ade/-His/-Leu/-Trp/X-α-Gal). Cotransformants with empty vectors pGADT7 and pGBKT7 were used as negative controls. A, adenine; H, His; L, Leu; T, Trp.