Figure 1.

KIN10 and TPS1 participate in Suc-induced hypocotyl elongation. A, Representative images of L. er. wild-type, KIN10-ox, and tps1 seedlings cultivated under a variety of photoperiods, with and without supplementation with 3% (w/v) Suc. All panels scaled identically. Images are a subset of seedlings used to generate data in (B) to (E). B to E, Lengths of hypocotyls of seedlings grown under (B and D) constant light, 16-h and 8-h photoperiods and (C and E) 4-h photoperiods. Photoperiods are indicated underneath graphs. F, Effect of Suc supplementation upon gin2-1 hypocotyl length. SEM is small under continuous light (0.03 mm to 0.05 mm), so is not visible on graphs. Data were analyzed with ANOVA and Tukey’s posthoc tests (n = 10 (B to E) or n = 20 (F) seedlings in three independent experiments; mean ± se). Different letters indicate statistically significant differences between means, specifically within each light condition (P < 0.05). B to E, MS is half-strength MS media, and Suc and Sor are 0.5 MS supplemented with 3% (w/v) Suc or equimolar sorbitol (87.6 mm osmotic control), respectively.