Figure 5.

COL12 down-regulates FT transcript levels without changing CO transcript levels. A, Transcript levels of the indicated flowering time genes in plants harvested at ZT 16. B, CO transcript levels in plants harvested at the indicated ZTs. C, FT transcript levels in plants harvested at the indicated ZTs. Transcript levels were analyzed by qRT-PCR in 12-d-old plants of the wild type and two independent transgenic 35S::HA-COL12 lines (COL12ox, #12 and #20). Plants were grown in long day with a white light fluence rate of 200 μmol m⁻² s⁻¹. Transcript levels were normalized to UBQ10 transcript levels. Error bars show the sem of 2 to 4 biological replicates (**P < 0.005, *P < 0.05 for difference to the wild type).