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COL12 does not affect CO protein levels. HA-CO and HA-COL12 levels were analyzed in two 35S::HA-COL12 lines (#16 and #20), a SUC2::HA-CO line, and the respective doubly transgenic SUC2::HA-CO 35S::HA-COL12 lines. Seedlings were grown in long day for 10 d and harvested at ZT16. Nuclear proteins were isolated and HA-CO and HA-COL12 proteins were detected using an α-HA antibody. Histone H3 levels served as a control. * indicates a nonspecific signal underlying one HA-COL12 signal.
