Figure 2.

Influence of organic acids on the activity of the AOX pathway in plant mitochondria. The effect of different organic acids on the activity of the AOX pathway in isolated mitochondria from Arabidopsis was analyzed. AOX activity was determined as described by Jacoby et al. (2015) using 5 mm citrate, isocitrate, 2-OG, fumarate, malate, pyruvate, or glyoxylate as effectors. Measurements were carried out as three independent biological replicates. Each biological replicate was measured twice, leading to a total of six values per column. The basal activity (no effector) of the AOX pathway was 3.96 ± 0.6 nmol oxygen min⁻¹ mg⁻¹ protein. Asterisks indicate that the differences (**, P < 0.01 and ***, P < 0.001) between the basal activity (no effector) and activities in the presence of the effectors are statistically significant as determined by two-way ANOVA with posthoc Tukey’s HSD test.