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. 2017 Dec 18;176(2):1850–1861. doi: 10.1104/pp.17.01566

Figure 3.

Figure 3.

TCP17 positively regulates IAA biosynthesis. A and B, Responses of Col-0 and 3tcp mutants to an auxin analog picloram (PIC). Col-0 and 3tcp seedlings were grown under Wc with different concentrations (0, 0.01, 0.1, 1.0, 2.0 μm) of PIC for 3 d and then transferred to Wc (A) or shade (B) for an additional 5 d before hypocotyl length was measured. C, TCP17 transgenic plants contain more free IAA than Col-0. Free IAA levels were analyzed in 7-d-old Col-0 and TCP17-OX transgenic seedlings grown under Wc. D, Shade induced free IAA accumulation is significantly impaired in triple mutant 3tcp. Col-0 and 3tcp were grown under Wc for 7 d and transferred to Wc or shade for 2 h before being collected for the measurements. Data shown are the average of three independent biological replicates and SE. *P < 0.05 and **P < 0.01; NS, not significant (P ≥ 0.05); based on Student’s t test.