Figure 6.

MVE morphology is altered in vps38 mutants. A to C, Root tip images of wild-type and vps38-expressing MVE marker, YFP-ARA7 (A); Golgi marker, YFP-SYP32 (B); and TGN marker, VHA-a1-GFP (C). Wild-type seedlings expressing each marker were incubated with either DMSO or 30 μm Wm for 1 h before observation under confocal microscope. Arrowheads indicate ring-shaped compartments. Graphs on the right illustrate the quantities of each marker in DMSO-treated wild type and vps38, and Wm-treated wild type (mean ± se; n > 11 regions of interest; *, 0.01 < P < 0.05; **, P < 0.01 by t test). D, Transmission electron micrograph showing MVEs and their ILVs of wild type, vps38-1, and vps38-2. Between 34 and 46 MVEs and between 36 and 47 ILVs from two independent roots were analyzed. Bars = 5 μm (A to C) or 500 nm (D). WT, wild type.