Figure 7.

Trafficking of endocytic cargo to the vacuole is hindered in vps38 mutants. A to C, Confocal microscopic images of wild-type and vps38 roots expressing PIN1-GFP (A), PIN2-GFP (B), or BRI1-GFP (C). Seedlings expressing each marker were incubated with either DMSO or 30 μm Wm for 1 h before observation under confocal microscope. D and E, Graphs illustrating the quantification of PIN2-GFP (D) and BRI1-GFP (E) signal intensities in the PM and intracellular region (mean ± se; n > 11; *, 0.01 < P < 0.05; **, P < 0.01 by t test). F, Images of wild-type and vps38 roots expressing PIN2-GFP in the presence of the endocytic tracer FM4-64. Seven-d-old seedlings were pulse-labeled with 5 μΜ FM4-64 for 2 h, washed 3 times, and incubated for 4 h before observation. G, Images of dark- or ConA-treated wild type or vps38 seedlings expressing PIN2-GFP. Seedlings were incubated with either DMSO, 1 μm ConA, or 30 μm Wm for 12 h before observation. Dark treatment was performed by incubating seedlings for 12 h in the dark. Bars = 5 μm. WT, wild type.