Figure 4.
Dependence of L. japonicus ethylene emission on compatible Nod factor. A, Ethylene emission over first 24 h following inoculation of L. japonicus Gifu with M. loti, M. loti nodC, or incompatible S. meliloti as indicated. B, Ethylene emission over first 24 h following treatment with purified M. loti Nod factor. C, Ethylene emission over 24 h for L. japonicus Gifu or the Ljein2a-2 Ljein2b-1 mutant. D, Ethylene emission over 24 h for L. japonicus Ljein2a-2 Ljein2b-1 mutant inoculated with M. loti. Plants were 5 days old at the time of treatment. Letters in A represent statistical differences determined by Tukey test at P < 0.05. Asterisks in B to D represent comparison of the two treatments by t test. *< 0.05, **< 0.01, ***< 0.001.