Table I. Fatty acid composition and accumulation of TAG in Chlamydomonas CC1010 cells subjected to high-light irradiation for 7 h (n = 3).
The values show the composition of fatty acids in percentage. The level of TAG is shown at the bottom. It was noted that the fatty acids specific to chloroplast lipids, namely 16:4 and 18:3(9,12,15), did not appreciably accumulate in TAG. As reported by Sakurai et al. (2014), 18:4 was supposed to be a mixture of 18:4(5,9,12,15) and 18:4(6,9,12,15), but, as reported by Sato et al. (2016), 18:4(6,9,12,15) was not detected in Chlamydomonas CC-1010.
| Fatty Acid | Total Lipids |
TAG |
||
|---|---|---|---|---|
| 0 h | 7 h | 0 h | 7 h | |
| 14:0 | 0.4 ± 0.1 | 0.4 ± 0.0 | 4.4 ± 0.7 | 2.2 ± 0.8 |
| 14:1 | 0.3 ± 0.1 | 0.2 ± 0.0 | 1.4 ± 0.3 | 0.9 ± 0.7 |
| 16:0 | 24.2 ± 1.8 | 24.7 ± 0.3 | 35.4 ± 2.5 | 30.6 ± 3.2 |
| 16:1(7) | 2.0 ± 0.4 | 2.5 ± 0.6 | 1.7 ± 0.1 | 2.0 ± 0.5 |
| 16:2(7,10) | 4.8 ± 1.0 | 3.3 ± 0.7 | 1.1 ± 0.3 | 2.1 ± 0.8 |
| 16:3(4,7,10) | 0.9 ± 0.2 | 0.9 ± 0.0 | 0.3 ± 0.3 | 0.4 ± 0.1 |
| 16:3(7,10,13) | 7.8 ± 0.3 | 7.7 ± 0.8 | 6.7 ± 0.8 | 6.9 ± 1.5 |
| 16:4(4,7,10,13) | 10.1 ± 2.3 | 10.0 ± 1.3 | 2.3 ± 0.2 | 3.3 ± 0.1 |
| 17:0 | 0.2 ± 0.1 | 0.1 ± 0.0 | 0.4 ± 0.4 | 0.4 ± 0.2 |
| 18:1(9) | 4.1 ± 0.2 | 5.1 ± 1.2 | 12.6 ± 1.9 | 16.1 ± 1.7 |
| 18:1(11) | 2.9 ± 0.7 | 3.0 ± 0.3 | 4.2 ± 1.0 | 3.9 ± 0.4 |
| 18:2(9,12) | 16.1 ± 2.8 | 14.7 ± 2.3 | 13.1 ± 1.3 | 14.5 ± 1.8 |
| 18:3(5,9,12) | 8.7 ± 0.5 | 9.0 ± 0.5 | 8.8 ± 1.1 | 7.3 ± 0.5 |
| 18:3(9,12,15) | 15.9 ± 1.9 | 16.9 ± 1.1 | 6.1 ± 0.6 | 8.0 ± 0.3 |
| 18:4(5,9,12,15) | 1.7 ± 0.2 | 1.6 ± 0.2 | 1.5 ± 0.4 | 1.7 ± 0.2 |
| TAG (% of total lipids) | – | – | 3.5 ± 0.2 | 7.8 ± 1.3 |