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. 2017 Dec 27;596(4):623–645. doi: 10.1113/JP275075

Table 1.

Diet composition of diets used to investigate the effect of reduced levels of dietary BCAAs in the context of a normal calorie diet

Amino acid defined diets Control AA ExLow AA ExLow BCAA Western (WD) WD + BCAA
Teklad diet number TD.140711 TD.140918 TD.150387 TD.88137 TD.150386
Colour Red Orange Blue Tan Green
Formula (g kg−1)
 l‐Alanine 9.38 2.18 12.1566
 l‐Arginine 6.3 1.46 6.3
 l‐Asparagine 20.58 4.79 22.6388
 l‐Aspartic acid 20.58 4.79 24.729
 l‐Cysteine 7.2 1.67 7.2
 l‐Glutamic acid 28.97 6.74 33.5548
 l‐Glutamine 33.77 7.87 36.0672
Glycine 2.96 0.69 5.2991
 l‐Histidine HCl, monohydrate 4.6 1.07 4.6
 l‐Isoleucine 7.8 1.81 1.81 8.8725
 l‐Leucine 25.4 5.9 5.9 15.6195
 l‐Lysine HCl 20.38 4.74 20.38
 l‐Methionine 6.7 1.56 6.7
 l‐Phenylalanine 6.6 1.54 6.6
 l‐Proline 7.41 1.72 10.9965
 l‐Serine 7.41 1.72 10.6844
 l‐Threonine 9.7 2.26 9.7
 l‐Tryptophan 3.4 0.79 3.4
 l‐Tyrosine 6.9 1.61 6.9
 l‐Valine 8.4 1.95 1.95 10.725
 dl‐Methionine 3.0 3.0
Casein 195 195
Sucrose 291.248 291.248 291.248 341.46 341.46
Corn starch 150.0 243.79 153.4368 150 114.683
Maltodextrin 150.0 243.79 153.4368
Anhydrous milkfat 210 210
Cholesterol 1.5 1.5
Corn oil 52.0 52.0 52.0
Olive oil 29.0 29.0 29.0
Cellulose 30.0 30 30 50 50
Mineral mix, AIN‐93M‐MX (94049) 35.0 35 35
Mineral mix, AIN‐76 (170915) 35 35
Calcium carbonate 4 4
Calcium phosphate Ca(H2PO4)2·H2O 8.2 8.2 8.2
Vitamin mix, Teklad (40060) 10.0 10.0 10.0 10.0 10.0
TBHQ, anti‐oxidant 0.012 0.012 0.012
Ethoxyquin, anti‐oxidant 0.04 0.04
Food colouring 0.1 0.1 0.1 0.1 0.1
% kcal from:
Protein (based on N × 6.25) 22 5.1 21.9 15.2 18.3
Carbohydrates 59.4 76.4 59.6 42.7 39.8
vFat 18.6 18.5 18.5 42 41.9
kcal g−1 3.9 3.9 3.9 4.5 4.6

Amino acid defined diets with the specified formulations were obtained from Envigo. These diets were used in the experiment outlined in Fig. 1 A and used to obtain the results shown in Figs 1, 2, 3.