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Subcutaneous transplantation of encapsulated MSCs in HA/alginate hydrogel with 1:1 w/w ratio: (a) Immunofluorescence staining for early neural differentiation markers GFAP (red) and β III-tubulin (green); and (b) pre-synaptic marker VAMP2 and the effects of presence of beta NGF. (c) The percentage of positive cells for VAMP2 antibody in each specimen according to immunostaining results of panel b. *P <0.05, NS: not significant.
