Skip to main content
. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: Cell Rep. 2018 Jan 30;22(5):1263–1275. doi: 10.1016/j.celrep.2017.12.104

Figure 3. TCR-CD3 and CD4 Mobility Decrease as TCR-pMHCII Affinity Increases.

Figure 3

(A–C) FRAP analysis of CD3δTG for 5c.c7+ CD3T+ CD4T+ M12 cells. Images were collected for 2-min postbleach at 3-s intervals. (A) Recovery trace represents mean ± SEM for the indicated number (n) of cells. (B) Mobile fraction (%) and (C) half-life (t1/2) for recovery were determined by curve fitting. Error bars indicate 95% confidence interval.

(D–F) FRAP of CD4TmCh. (D) Recovery trace, (E) mobile fraction (%) and (F) half-life (t1/2) as presented in (A)–(C).

# denotes values with poor fitting with an exponential function. Results shown are representative of at least two experiments. See also Figure S3.