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. 2018 Feb 15;27(4):252–261. doi: 10.1089/scd.2017.0168

FIG. 4.

FIG. 4.

Wnt/β-catenin pathway was activated by Sal B during differentiation. (A) Relative expression of genes from Wnt ligands was determined by qPCR at day 14 of differentiation in the treated and untreated cells. (B) Expression of Wnt1, cyclin D1, c-Myc, Numb, and β-catenin was detected by western blot in the treated and untreated cells. (C, D) Wnt1 and cyclin D1 protein expression was measured using histogram normalized to GAPDH after calculation based on the results of western blot. (E) The amount of nuclear beta-catenin protein was measured using histogram normalized to histone protein after calculation based on the results of western blot. (F) Relative expressions of LEF1, TCF3, Numb, and cyclin D1 were determined by qPCR in the treated cells and compared to the untreated cells at day 14 of differentiation. Data represent mean ± SEM. *P < 0.05, **P < 0.01. LEF, lymphoid enhancer-binding factor; TCF, T cell factor.