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. Author manuscript; available in PMC: 2018 Jun 18.
Published in final edited form as: Nat Struct Mol Biol. 2017 Dec 18;25(1):109–114. doi: 10.1038/s41594-017-0006-4

Figure 1. RNA-DamID accurately detects lncRNA-chromatin interactions in vivo.

Figure 1

(a) Schematic representation of RNA-DamID. A lncRNA of interest (red) tagged with 3xMS2 stem loops is co-expressed with a Dam-MCP fusion protein (blue and green, respectively) under the spatial and temporal control of GAL4 (purple). The Dam-MCP fusion is recruited to sites of lncRNA-chromatin interaction and methylates adenines within the sequence GATC. lncRNA binding sites are identified genome-wide by DamID. (b) A UAS-3xMS2-roX2 transgene was inserted on chromosome 3L. (c) Fold enrichment of roX2 RNA-DamID binding in whole male larvae, normalised to the negative control, reveals binding exclusively to the X chromosome (average of two biological replicates).