Fig. 3.

Axons from central complex lineages innervate unique combinations of commissural fascicles in the embryonic grasshopper brain. Panels represent concatenated confocal images of brain slices at four depths (a, e, i: 80–88μm; b, f, j: 90–98μm; c, g, k: 100μm; and d, h, l: 107μm) as measured from the ventral surface of the brain (according to the neuraxis) following 8B7 immunolabeling at 50% of embryogenesis (e50%) and prior to formation of the fan-shaped body. Axon projections (white) from the Z (a – d), Y (e – h) and X (i – l) tracts have been individually traced into the commissural system. Nomenclature of commissural fascicles follows that for the adult brain (Boyan et al. 1993). Scale bar represents 40μm