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. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: ACS Chem Neurosci. 2016 Nov 23;8(3):486–500. doi: 10.1021/acschemneuro.6b00221

Figure 7.

Figure 7

Time course of DA-, SK609-, and SK213-induced ERK1/2 phosphorylation at CHO-D3R. (A) Representative Western blot of ERK1/2 phosphorylation induced by DA, SK609, and SK213 at the indicated times. CHO-D3R cells were pretreated at 0, 1, 2, 5, 10, 20, 30, 60, and 240 min with 1 μM of DA, SK609, or SK213, washed once with PBS buffer and lysed for Western blots as described in Methods. (B) Signals were quantified by densitometry and normalized to total ERK. The graphs represent mean ± SEM of three independent experiments. *P < 0.05 compared with DA by one-way ANOVA followed by Newman–Keuls multiple-comparison test.