Skip to main content
. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: ACS Chem Neurosci. 2016 Nov 23;8(3):486–500. doi: 10.1021/acschemneuro.6b00221

Figure 9.

Figure 9

SK609 and SK213 do not recruit β-arrestin-2 for signaling at D3R. Dose–response curve of PD128907, SK609, and SK213 for recruiting β-arrestin-2 at D3R was tested. PathHunter eXpress β-arrestin-2 U2OS cells stably expressing hD3R were plated at 10 000 cells/well in a 96-well plate, incubated for 48 h, and stimulated for 90 min with vehicle and indicated concentrations of compounds PD128907, SK609, and SK213 as described in Methods. Following stimulation, signal was detected using the PathHunter detection reagents following the manufacturer’s protocol. RLU (relative luminescence units) represents a measure of β-arrestin-2 recruitment in this assay, and the data is presented as net change of RLU minus vehicle control (0.027 ± 0.002) of three independent experiments performed in triplicate.