Table 3.
Ki Values (nM) of SK609 and Its Analogue or Reference Compound Binding to the CHO-D3Ra
compd |
Ki (nM) to CHO-hD3
|
|
---|---|---|
Ki-H | Ki-L | |
DA | 9.9 ± 4.4 | 246 ± 105 |
PD128907 | 3.7 ± 1.6 | 14.6 ± 3.6 |
609 | 283 ± 30 | 4955 ± 1314 |
608 | 103 ± 25 | 1029 ± 45 |
32 | 1109 ± 310 | 9504 ± 1551 |
52 | 1758 ± 901 | 9293 ± 1015 |
213 | 3708 ± 1003 | 8082 ± 1185 |
232 | 7008 ± 1850 | 7705 ± 1987 |
Competitive inhibition by SK609 and its analogues or reference compounds of [3H]methylspiperone binding to D3R was conducted. The competition curves were fitted by two-site analysis and its high-affinity Ki values (Ki-H) and low-affinity Ki values (Ki-L) were determined using the Prism program. Each value represents mean ± SEM of three experiments performed in duplicate.