Figure 3. CMV and CAG promoter comparison.

Plot of reporter fluorescence. Primary neuronal cultures grown in 384-well plates were infected with reporter-containing AAV virus added at 1 DIV. The indicated peptides were added at 5 DIV and were present throughout the experiment. A. The effect of Aβ42 on the CMV promoter expressing the dsRed fluorescent protein. B. The effect of Aβ42 on the CAG promoter expressing tdTomato. n indicates number of wells used for each condition and the error bars represent standard error of the mean (SEM). Statistical significance for the difference in fluorescence between Aβ42 and scrambled Aβ conditions on the last day of measurement was calculated by an unpaired t-test.