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. 2018 Feb 15;13(2):e0192404. doi: 10.1371/journal.pone.0192404

Fig 2. Euglena extract represses the gene expression of master regulators of adipocyte-differentiation.

Fig 2

Relative gene abundances of (A) PPARγ and (B) C/EBPα in hASCs after treatment with no additive (control) or 20% Euglena extract for Day 3, Day 7, or Day 14 were monitored by RT-qPCR. Data represent mean ± SEM (n = 3). *P < 0.05 vs. control (Student’s t-test). (C) Western blotting analysis of PPARγ, C/EBPα, and GAPDH (as internal control). Cell lysate (25 μg) derived from soluble fraction was loaded in each lane. The data shown in (C) are representative from 3 independent experiments. Day 0 shown in (A)-(C) means before induction of adipocyte-differentiation.