Skip to main content
. 2018 Feb 15;13(2):e0193101. doi: 10.1371/journal.pone.0193101

Fig 4. Effects of P12-3A on cultured skin cells.

Fig 4

A, Insulin concentrations as a function of time in logarithmically growing primary murine skin fibroblasts in the absence or presence of P12-3A (100 μM). Note that insulin levels remain constant in the presence of P12-3A, reflecting both the effectiveness of the peptide inhibiting insulin degradation and also the stability of the peptide in biological milieu. Data are mean ± SEM of 4 independent replications. *P<0.05, **P<0.01. B, Proliferation of cells in the absence or presence of P12-3A (100 μM). Data are mean ± SEM of 6 independent replications. No significant differences were observed. C,D,E, P12-3A (100 μM) potentiates insulin-induced collagen production in skin fibroblasts. Collagen production was assessed by COL1A1 mRNA levels (C), levels of hydroxyproline secreted into the medium (D), and cell-associated mature alpha-1 type I collagen levels detected by Western blotting (E). Data are mean ± SD of 4 independent replications. *P<0.05, **P<0.01. F, P12-3A (100 μM) potentiates the migration of keratinocytes in a scratch wound assay. Migration of HaCaT cells 48 h after induction of a scratch wound in the presence of the indicated quantities of insulin and/or P12-3A (100 μM). Data are mean ± SEM of 6 independent replications. *P<0.05, **P<0.01.