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. 2018 Feb 15;13(2):e0193101. doi: 10.1371/journal.pone.0193101

Table 1. Peptide sequences synthesized and their potency in activity assays with FRET1 and insulin.

Name N-term Sequence C-term Ki FRET1 (μM) Ki Insulin (μM)
C7C-1 NH3+ ACSWWSIHLCG COO- 112 ± 6 3.7 ± 0.7
C7C-1A NH3+ ACSWWSIHLCG amide >100 >10
C7C-1B NH3+ ACSWWSIHLCGGG COO- >100 >10
C7C-2 NH3+ ACNAGHLSQCG COO- >500 >10
C7C-2A NH3+ ACNAGHLSQCG amide >500 >10
P12-1 NH3+ VHWDFRQWWQPS COO- 7.7 ± 0.7 0.8 ± 0.04
P12-1A NH3+ VHWDFRQWWQPS amide 5.0 ± 0.3 1.3 ± 0.2
P12-1B NH3+ VHWDFRQW amide 41 ± 6.4 7.0 ± 1.7
P12-1C acetyl FRQWWQPS COO- 139 ± 21 >10
P12-1D acetyl WDFRQWWQ amide 140 ± 28 >10
P12-2 NH3+ LNFPMPSRPHSS COO- >100 >10
P12-2A NH3+ LNFPMPSRPHSS amide >100 >10
P12-2B NH3+ LNFPMPSR amide >500 >10
P12-2C acetyl MPSRPHSS COO- >500 >10
P12-2D acetyl FPMPSRPH amide >500 >10
P12-3 NH3+ QSLPWCYPHCVT COO- 8.9 ± 0.3 3.9 ± 1.6
P12-3A NH3+ QSLPWCYPHCVT amide 10 ± 0.4 4.1 ± 0.3
P12-3B NH3+ QSLPWCYP amide 39 ± 20 >10
P12-3C acetyl WCYPHCVT COO- 35 ± 4.7 >10
P12-3D acetyl LPWCTPHC amide 102 ± 14 >10
P12-4 NH3+ WSPISGKFFQRF COO- 3.9 ± 0.5 1.5 ± 0.3
P12-4A NH3+ WSPISGKFFQRF amide 4.2 ± 1.3 2.6 ± 0.7
P12-4B NH3+ WSPISGKF amide >500 >10
P12-4C acetyl SGKFFQRF COO- >500 >10
P12-4D acetyl PISGKFFQ amide >500 >10