Fig 3. EBS reduces testicular inflammatory response and leucocyte infiltration.

AG129 mice subcutaneously infected in the footpad with 5x10⁴ PFU/mouse of ZIKV were intraperitoneally treated with EBS (10 mg/kg body weight/mouse/day) or solvent control on day 1–6 after infection. Testes and sperm were collected on 9 dpi. (A) Cytokine profile of seminal fluid on 9 dpi. Cytokine levels were evaluated by ELISA array. Data are mean ± standard deviation (n = 5 mice/group). A two-tailed 0.01 significance level; a (Mock vs. ZIKV) and b (ZIKV vs. ZIKV+EBS) by Kruskal-Wallis, Bonferroni Post Hoc Test. (B-C) Confocal microscopy of testes sections immunostained for CD45 (green, B), IL-1β (red, C), and Hoechst for nuclei (blue). Scale bar: 100 μm.