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. 2018 Feb 15;14(2):e1006854. doi: 10.1371/journal.ppat.1006854

Fig 4. Antioxidant EBS alleviates ZIKV-associated testicular pathology.

Fig 4

AG129 mice subcutaneously infected in the footpad with 5x104 PFU/mouse of ZIKV were intraperitoneally treated with EBS (10 mg/kg body weight/mouse/day) or solvent control on 1–6 dpi. Testes and semen were collected on 9 dpi. (A) Histology of testes sections stained with haematoxylin and eosin. Arrows indicate lumen (green), sperm (red), blood capillary (blue), interstitial cell (yellow), and degeneration of SNT (black). Scale bar: 200 μm. (B) Confocal microscopy of testes sections immunostained for TRA98 (green, germ cells), ETV5 (red, blood—testis barrier), and Hoechst for nuclei (blue). Differential interference contrast (DIC). Scale bar: 100 μm. (C) Total number of sperm (cell/ml). (D) Proportion of sperm with normal and abnormal morphology. Data are mean ± SD (n = 6 mice/group). **P<0.01 by Kruskal-Wallis, Bonferroni post-hoc test. (E) Confocal microscopy of sperm morphology with CellTracker staining for cytoplasm (red) and Hoechst for nuclei (blue). Scale bar: 100 μm. Arrows indicate sperm with abnormal morphology.