(
A) Bar chart representing the reproducibility of proteins identified in several biological replicates. Proteins identified in only one replicate were discarded and not analyzed further. (
B) Newly-synthesized proteins in each treatment group were classified according to their localization in cellular compartments using Gene Ontology. (
C) Distribution of non-normalized, raw protein intensities showing similar protein expression in all conditions. (
D–F) Correlation plots of protein intensities across biological replicates for the 2 hr datasets for each treatment, TTX, bicuculline or untreated (control), respectively. (
G) Correlation plots of protein intensities for the data sets acquired in this study (2 hr) as well as the data sets in a previous study (
Schanzenbächer et al., 2016) that is further analyzed here. In order to be plotted here, the protein must be identified in the two experiments being compared. (
H) The unique proteins identified in a single treatment group, showing the number of replicates in which the protein was identified. (
I) The methionine content of the peptides identified in our study, proteins identified in our study and the global proteome (from UniprotKB).