Figure 3. Anti-miR-17 therapy impeded MYC-driven tumorigenesis.

A. Schematic representation of experimental methods. MYC HCCs were induced by activating MYC at 4 weeks of age. Mice were treated with control or Anti-miR-17 oligonucleotide once tumor volume of 50 mm3 was reached. Mice were imaged every week with MRI and tumor volume measured. Mice were sacrificed after they received 4 doses of therapy at weekly intervals. B. Serial MRI images of representative mouse from control and anti-miR 17 groups showing delayed tumor progression in latter group. Red arrows point to the tumors. C. Tumor volume from mice treated with control of anti-miR-17 oligonucleotide at week 4 was normalized to tumor volume at week one. At week 4, tumors were larger in the control group than in the anti-miR-17 group. Also, the growth curve of the tumors from week one to week 4 has been plotted to demonstrate the difference. D. Gross morphology of liver tumors after 4 doses of treatment shows that mice treated with control oligonucleotide had larger tumors and more numerous tumors than those treated with anti-miR-17 therapy.