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. 2017 Dec 24;9(5):5588–5599. doi: 10.18632/oncotarget.23652

Figure 6. Effects of aging and CR on STAT3 activation and STAT3 inducing cytokines.

Figure 6

(A) Phosphorylated (activation form) STAT3 is detected by Western blotting in the nuclear fraction of kidney. The blots were quantified by densitometry (n = 8). TFIIB was used as the loading control. (B) The blots in figure 6A were quantified by densitometry (n = 8). #P < 0.05 vs young. *P < 0.05 vs old. (C) Gene expressions of IL-19, IL-24, and LIF were detected by qPCR. mRNA levels were normalized to the 18S level. #P < 0.05 vs young. *P < 0.05 vs old.