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. 2017 Dec 14;9(5):6015–6027. doi: 10.18632/oncotarget.23210

Figure 1. E7 expression by keratinocytes alters their susceptibility to killing by CTL.

Figure 1

Primary KC were isolated from B6 or E7 transgenic mice and loaded with SIINFEKL peptide. EGFP+OT-1 T cells were isolated and co-cultured with skin cells, with indicator dye for activated caspases. (A) KC survival over 30 hours of co-culture. Average of 4 experiments shown, error bars represent SD. (B) The percentage of KC deaths at 5 hour intervals was determined by counting newly dead cells at each time point and expressing as a fraction of the total number of cells in each frame. (C) KC death at 30 hours in co-culture with effector cells (black) or in monoculture (grey). (D) KC were incubated with Z-DEVD-FMK or DMSO (Mock) 60 minutes before and during co-culture; death assessed at 30 h. (E) CTL and KC co-cultures at 13 h showing attachment of CTL (green) to KC (arrow), and at 30 h showing early apoptosis of KC as indicated by red color change. Bar is 10 μm. See also, Supplementary Video 1. (F) Duration of attachments of E7-expressing (E7) or non-transgenic (B6) KC with CTL while incubated with DMSO (mock), Z-DEVD-FMK, or without peptide loading. (*p<0.05; n.s. not significant).