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. 2017 Dec 14;9(5):6015–6027. doi: 10.18632/oncotarget.23210

Figure 2. CD8 T cells kill E7KC by perforin-independent mechanisms.

Figure 2

In vivo and in vitro activated OVA-specific EGFP+Prf-competent and EGFP+Prf1-/- CD8 T cells were added to KC primary cultures. (A) Flow cytometry of wild-type (WT) and perforin-deficient (Prf1-/-) CD8 T cells pre-gated on size and granularity, and CD8 expression. (B) Percentage of CD44+ cells after gating. Results of 4 independent CD8 harvests. (C) Primary KC from E7 and B6 mice were loaded with SIINFEKL and co-cultured with GFP+Prf1-/- CD8 T cells. KC survival calculated as in Figure 1. (D) The final frames of movies were used to calculate percentage KC death over 30 hours. (E) Frequency distribution of the duration of attachments of effector cells to targets at 30-minute intervals. (F) Quantification of T cell death in co-cultures with antigen-loaded E7-expressing or non-transgenic KC at 30 hours. (*p<0.05; n.s. not significant).