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. 2017 Dec 14;9(5):6015–6027. doi: 10.18632/oncotarget.23210

Figure 3. Perforin-competent CD8 effector T cells require TNFα but not IFNγ for cytotoxic function.

Figure 3

EGFP+OT-1 T cells were incubated with KC loaded with SIINFEKL. (A-C) An inhibitory antibody to TNFα was added to the KC before and during co-culture. KC death (A), duration of attachment of effectors to targets (B) and death of effector cells (C) with anti-TNFα or isotype control antibody. (D-F) Anti-IFNγ antibody was added to KC before and during co-culture. (D) KC death, duration of attachment (E) of effectors to targets and effector cell death (F) with anti-IFNγ or isotype control antibody. (G-H) Effector cells were pre-treated with antibody against TNFα (E) or IFNγ (F) before co-culture with target KC. Averages of 5 imaging sites of duplicate wells are shown as a single dot. Each dot is one separate experiment. Error bars are SEM. (*p<0.05).